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IPFS News Link • Science, Medicine and Technology

CRISPR gene editing is undergoing rapid development and improvement

• nextbigfuture.com

Indeed, Chinese scientists recently began a human clinical trial using CRISPR-edited cells to fight lung cancer, and U.S. clinical trials are slated to begin in 2017. But leading up to this exciting milestone, researchers performed some editing on the CRISPR system itself.

Strategies used to modulate Cas9 activity. (Computational and Structural Biotechnology Journal - Programmable Genome Editing Tools and their Regulation for Efficient Genome Engineering)
(a) Group II intron (GII)-based switch,
(b) separating Cas9 into two peptides, termed split-Cas9,
(c) Tetracycline-inducible and reversible expression system, and
(d) ligand-dependent dimerization of split-Cas9.
(e) Light-dependent dimerization of split-Cas9, termed photoactivatable Cas9 (paCas9),
(f) intein-Cas9, which are activated by splicing of a ligand-dependent intein,
(g) and unstable destabilizing domain-Cas9 (DD-Cas9) fusions, which are degraded unless provided with the ligand, Shield.
 


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