California's Secretary of State Alex Padilla announced the ballot proposal had been cleared.
The latest measure would ask voters in 2020 to decide whether to open up a secession discussion. If passed, a second election would be held a year later asking voters to affirm the decision and become an independent country.
Advocates have until mid-October to gather 365,880 signatures of registered voters to get it on the ballot.
Marcus Ruiz Evans and Louis J. Marinelli, co-founders of the group Yes California, said the second vote would show that Californians are serious about secession and would strengthen the case for foreign governments to recognize the state's independence.