The One's chestpiece features a simple electronic display indicating battery life, volume and frequency. Utilizing its standard 3.5-mm headphone jack, users can plug in a set of phones of their choice, although the standard package does include a set of earbuds. The headphone jack also serves as the battery-charging port, with one or two charges of the lithium-ion battery (via a USB charger) being required per week, depending on the amount of use.

Using the Thinklabs Stethoscope app (iOS, Android, Mac or PC), doctors are able to make and then selectively filter audio recordings in order to better listen for specific conditions. They can also slow down and isolate specific sounds, or share recordings with other physicians.